The people behind the protocol.

Dr. John Hughes, D.O. practices osteopathic, integrative, and regenerative medicine. His clinic, Aspen Integrative Medicine, Inc., provides the latest innovations in modern and natural medical care in Basalt and Aspen, Colorado. Dr. Hughes graduated from the Arizona College of Osteopathic Medicine in 2007 and trained in family practice at the University of Arizona. He has specialized in integrative and regenerative medicine, prolotherapy, platelet rich plasma (PRP) therapy, and osteopathic manual medicine since 2009.

In 2013, Dr. Hughes gained an interest in the treatment of traumatic brain injuries after, incidentally, treating a patient with a severe TBI using a regenerative medicine protocol of hyperbaric oxygen, an intranasal PRP/insulin/nutrient cocktail, IV nutrition, cranial therapy, and autologous stem cells. In 2014, Dr. Hughes founded TBI Therapy, LLC, a TBI clinic in Colorado focused on the development of specific protocols for mild to severe brain injury patients.

In 2016, Dr. Hughes received a patent for his TBI Therapy Protocol and intranasal cocktail formulation. His TBI rehabilitation work has been recognized by the U.S. military, the regenerative medical community, brain injury specialists, and patients. Dr. Hughes sees patients from around the U.S. on a regular basis in Basalt, Colorado through TBI Therapy, his second company.

Thoryn Stephens is the Founder and CEO of BRAIN.ONE, an AI-powered brain fitness and health platform designed to enhance cognitive performance through science-backed protocols, micro-habits, biometric tracking, and personalized behavioral insights.

Bridging a foundational background in biotechnology and molecular biology with two decades of expertise in data science and digital innovation, Thoryn has built a dynamic career at the intersection of health, technology, and consumer behavior. He began his career in scientific research, contributing to drug development efforts targeting cardiovascular and infectious diseases. Since then, he has held strategic leadership roles at top-tier organizations, including Cytokinetics (in partnership with GlaxoSmithKline), Unilever Prestige, and Fox Networks, where he drove enterprise growth through advanced analytics, predictive modeling, and AI-driven technology solutions.

A recognized thought leader and dynamic speaker, Thoryn has delivered keynotes at prestigious institutions and global conferences, including The Wharton School of the University of Pennsylvania, WIRED London, Cannes Lions, and AdTech Tokyo.

A lifelong advocate of human optimization, Thoryn applies his professional commitment to peak performance directly to his personal life. Based in Aspen, Colorado, he is a competitive triathlete and an avid skier who logs over 100 days on the mountain each year.